qPCR,定量PCR,是目前基因表达定量公认的金标准实验方法。
标准规范的qPCR实验包含引物设计合成,引物及实验体系验证,目标产物验证,上机及数据分析等多个步骤。
InstantPCR基因表达定量PCR试剂盒,采用SYBR Green I实时荧光PCR进行基因表达定量。
试剂盒包括验证过的定量PCR引物和PCR MasterMix,加入样本就可以上机实验。
Fig.1 PCR扩增曲线
Fig.2 DNA溶解曲线
Fig.3 DNA凝胶电泳
Fig.4 PCR扩增效率统计
案例1:非小细胞肺癌耐药性相关研究
Integrated analysis of DNA methylation and mRNA expression profiling reveals candidate genes associated with cisplatin resistance in non-small cell lung cancer. Epigenetics, 2014. IF: 5.108.
案例2:肝癌相关细胞凋亡研究
Apolipoprotein A1: a novel serum biomarker for predicting the prognosis of hepatocellular carcinoma after curative resection. Oncotarget, 2016. IF: 5.008.
案例3:肺癌AKT信号通路相关研究
Epigenetic downregulation of RUNX3 by DNA methylation induces docetaxel chemoresistance in human lung adenocarcinoma cells by activation of the AKT pathway. The International Journal of Biochemistry & Cell Biology, 2013. IF: 4.24.
案例4:缺氧标志基因相关研究
Iron Regulatory Protein 1 Suppresses Hypoxia-Induced Iron Uptake Proteins Expression and Decreases Iron Levels in HepG2 Cells. J Cell Biochem, 2015. IF: 3.446.
案例5:宫颈癌相关研究
Lu X, Lin Q, Lin M, et al. Multiple-integrations of HPV16 genome and altered transcription of viral oncogenes and cellular genes are associated with the development of cervical cancer. Plos One, 2014. IF: 3.234.
案例6:淋巴瘤相关细胞凋亡研究
Targeting protein neddylation with an NEDD8-activating enzyme inhibitor MLN4924 induced apoptosis or senescence in human lymphoma cells. Cancer Biology & Therapy, 2015. IF: 3.072.
案例7:白血病相关HOX基因研究
Qiu Y, Song B, Zhao G, et al. Expression level of Pre B cell leukemia homeobox 2 correlates with poor prognosis of gastric adenocarcinoma and esophageal squamous cell carcinoma. International Journal of Oncology, 2010. IF: 3.025.
案例8:非小细胞肺癌中miRNA相关研究
EGCG Enhances the Efficacy of Cisplatin by Downregulating hsa-miR-98-5p in NSCLC A549 Cells. Nutrition & Cancer, 2014. IF: 2.47.